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KMID : 1094720100150020341
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Biotechnology and Bioprocess Engineering
2010 Volume.15 No. 2 p.341 ~ p.348
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Effects of Cell-seeding Methods of Human Osteoblast Culture on Biomechanical Properties of Porous Bioceramic Scaffold
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Jung Duk-Young
Yamada Takashi
Tsuchiya Toshie
Ryu Su-Chak
Han Dong-Wook
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Abstract
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Many studies have been performed to accelerate osteoinduction and osteoconduction into porous ceramic scaffolds by seeding them with cells. In this study, we compared available cell-seeding methods on a porous β- tricalcium phosphate (β-TCP) scaffold and evaluated the effects of cell-seeding on the mechanical properties of the porous β-TCP scaffold. Three types of porous bioceramic scaffolds were used: dry scaffold, scaffold wetted with media, and scaffold cultivated with normal human osteoblasts (NHOs). Cell-seeding into the porous β-TCP scaffolds was performed by conventional, centrifuge, high-density, and vacuum methods. After confirming cell proliferation with MTT assay and cell staining, a compressive test was performed after 2 and 4 weeks of cell culture. The vacuum method based on the high-density cell culture inserted effectively NHOs into the β-TCP scaffolds. The compressive elastic modulus of wetted β-TCP scaffolds decreased significantly ( p < 0.05) about 20~30% after 2 and 4 weeks of incubation in comparison with that of the dry scaffold. However, the compressive strength of the scaffolds cultivated with NHOs for 3 weeks was significantly ( p < 0.05) higher than that of scaffolds without NHOs. The vacuum with the high-density of cell-seeding seems to be a suitable method for seeding cells into complex porous ceramic scaffolds. Cell proliferation and uniform distribution in the scaffolds can change the initial mechanical properties of porous ceramic scaffolds.
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KEYWORD
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Porous β-tricalcium phosphate scaffold, human osteoblasts, cell-seeding method, vacuum, mechanical property
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